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Published Articles >> Table of Contents >> Abstract
2005 IEEE Computational Systems Bioinformatics Conference (CSB'05)
pp. 208-211
Deformable Modeling for Improved Calculation of Molecular Velocities from Single-Particle Tracking
Peter M. Kasson, Stanford University
Mark M. Davis, Stanford University
Axel T. Brunger, Stanford University
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DOI Bookmark: http://doi.ieeecomputersociety.org/10.1109/CSB.2005.28
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| Abstract |
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Single-particle tracking provides a powerful technique for measuring dynamic cellular processes on the level of individual molecules. Much recent work has been devoted to using single particle tracking to measure long-range movement of particles on the cell surface, including methods for automated localization and tracking of particles [1-3]. However, most particle tracking studies to date ignore cell surface curvature and dynamic cellular deformation, factors frequently present in physiologically relevant situations. In this report, we perform quantitative evaluation of single-particle tracking on curved and deforming cell surfaces. We also introduce a new hybrid method that uses non-rigid cellular modeling for improved computation of single-particle tracking trajectories on the surfaces of cells undergoing deformation. This method combines single-molecule and bulk fluorescence measurements in an automated manner to enable more accurate and robust characterization of dynamic cell physiology and regulation.
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 Additional Information
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Citation:
Peter M. Kasson, Mark M. Davis, Axel T. Brunger,
"Deformable Modeling for Improved Calculation of Molecular Velocities from Single-Particle Tracking,"
csb,
pp. 208-211,
2005 IEEE Computational Systems Bioinformatics Conference (CSB'05),
2005
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